Journal: medRxiv

Article Title: Acetate-producing Prevotella copri aggravates schizophrenia behaviors by modulating serotonin metabolism

doi: 10.1101/2025.03.25.25324621

Figure Lengend Snippet: Increasing acetate intake regulated gut-brain serotonin metabolism. a Mice experimental design. b Body weight changes before behavioral experiments (n = 10 per group). c The effect size of percent PPI impairments at 75, 80 and 85 dB. The percent scores of distances ( d ) and time ( e ) spent in target quadrant of MWM test. f-q Western blot results of proteins expression in colon ( f ) and PFC ( g ). The quantification of Tph1 ( h ), Ddc ( i ), Maoa ( j ), Sert ( k ), Ffar3 ( l ) and Ffar2 ( m ) in the colon as well as Tph2 ( n ), Ddc ( o ), Maoa ( p ) and Sert ( q ) in the PFC were performed. Data are represented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns depicts no significant difference.

Article Snippet: The information for antibodies is as follows: Tph1 (D10C10) Rabbit mAb (#12339, Cell Signaling Technology), Tph2 (D3E5I) XP® Rabbit mAb (#51124, Cell Signaling Technology), Ddc (D6N8N) Rabbit mAb (#13561, Cell Signaling Technology), Maoa (E3L3B) Rabbit mAb (#75330, Cell Signaling Technology), anti-serotonin transporter antibody (EPR26259-61, abcam), FFAR3 antibody (CSB-PA008939, Cusabio Technology), FFAR2 Polyclonal antibody (19952-1-AP, Proteintech Group), β-Actin (13E5) Rabbit mAb (#4970, Cell Signaling Technology).

Techniques: Western Blot, Expressing

Journal: Brain research bulletin

Article Title: Electroacupuncture and Tongbian decoction ameliorate CUMS-induced depression and constipation in mice via TPH2/5-HT pathway of the gut-brain axis.

doi: 10.1016/j.brainresbull.2025.111207

Figure Lengend Snippet: Fig. 6. EA þ TB improved 5-HTP content in the brain-gut axis of CUMS mice. (a) 5-HTP levels in the PFC via ELISA. (b) 5-HTP levels in the colon via ELISA. (c) 5-HTP concentration in serum (ELISA). (d) WB analysis of TPH1 expression in the colon. (e) Relative expression data of TPH1 protein in the colon. (f) Relative mRNA expression of TPH1 in the colon. Kruskal-Wallis test, adjusted for false discovery rate (FDR), was employed to conduct multiple comparisons among five groups. Asterisks denote the following significance levels: * indicates p < 0.05, * * indicates p < 0.01, * ** indicates p < 0.001, and * ** * indicates p < 0.0001. Panels a, b, c, f: n = 5 mice per group; panels d, e: n = 4 mice per group.

Article Snippet: Following a 2-hour incubation at room temperature with 5 % skimmed milk for blocking, the membrane was incubated overnight at 4◦C with specific primary antibodies targeting TPH2 (1:2000, A14520, ABclonal), TPH1 (1:1000, 12339S, CST), and GAPDH (1:5000, GB15004–100, Servicebio).

Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Cinnamic Acid, Perillic Acid, and Tryptophan Metabolites Differentially Regulate Ion Transport and Serotonin Metabolism and Signaling in the Mouse Ileum In Vitro

doi: 10.3390/ijms25126694

Figure Lengend Snippet: Effects of different phytochemicals and Trp metabolites on the gene expression of 5-HT metabolism-related enzymes, including Tph1 ( A ) and Maoa ( B ) in the ileum of mice. The data in the charts are the means ± SEMs. * p < 0.05, ** p < 0.01, and *** p < 0.001 compared to the control group. Tph1, tryptophan hydroxylase 1; Maoa, monoamine oxidase A; VMA, vanillylmandelic acid; PA, perillic acid; MurA, muramic acid; CA, cinnamic acid; CGA, chlorogenic acid; NAS, N -acetylserotonin; ILA, indolelactic acid; IAld, indoleacetaldehyde. The number of animals is shown in .

Article Snippet: Primary antibody against Tph1 (Cat# 12339) was purchased from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Gene Expression, Control

Journal: International Journal of Molecular Sciences

Article Title: Cinnamic Acid, Perillic Acid, and Tryptophan Metabolites Differentially Regulate Ion Transport and Serotonin Metabolism and Signaling in the Mouse Ileum In Vitro

doi: 10.3390/ijms25126694

Figure Lengend Snippet: Effects of different phytochemicals and Trp metabolites on the protein abundance of tryptophan hydroxylase 1 ( Tph1 ) in the ileum of mice. The data in the charts are the means ± SEMs, n = 3. CA, cinnamic acid; PA, perillic acid; IAld, indoleacetaldehyde; NAS, N -acetylserotonin.

Article Snippet: Primary antibody against Tph1 (Cat# 12339) was purchased from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Quantitative Proteomics